KMID : 0379119740020010025
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Korean Journal of Mycology 1974 Volume.2 No. 1 p.25 ~ p.29
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Studies on the Cellulase of Penicillium sp . Isolated from Soils (2)
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Abstract
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1. Penicillium sp. C 13-13 strain was obtained with the treatment of mutagenic agents(N.T.G.) and by single spore isolation method from the Penicillium sp. C8-14 strain, which was reported in the previous paper.
2. The above strain had a few spores and to obtain seed culture, it was cultured at 30¡É and initial pH 4.5¡5.0, with air rate 6§¤/min., and agitation 600 rpm for 48 hours in 10% wheat bran medium in 20§¤- Jar fermenter.
When the broth that had above 70§¢ of mycelium was inoculated into wheat bran medium and incubated at 29¡33¡É for 72 hrs, the cellulase activity of the koji was higher.
3. Adding calcium chloride and magnesium sulfate to the wheat bran medium to 1.5% and 0.015% respectively, the cellulase activity of the koji was higher than that of the control.
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